Publication Date:
1996-12-13
Description:
Activation of the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors is implicated in the pathophysiology of traumatic brain injury. Here, the effects of mechanical injury on the voltage-dependent magnesium (Mg2+) block of NMDA currents in cultured rat cortical neurons were examined. Stretch-induced injury was found to reduce the Mg2+ blockade, resulting in significantly larger ionic currents and increases in intracellular free calcium (Ca2+) concentration after NMDA stimulation of injured neurons. The Mg2+ blockade was partially restored by increased extracellular Mg2+ concentration or by pretreatment with the protein kinase C inhibitor calphostin C. These findings could account for the secondary pathological changes associated with traumatic brain injury.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, L -- Rzigalinski, B A -- Ellis, E F -- Satin, L S -- HL07537-14/HL/NHLBI NIH HHS/ -- NS 27214/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1996 Dec 13;274(5294):1921-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Post Office Box 980524, Richmond, VA 23298-0524, USA. lsatin@gems.vcu.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8943207" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Brain Injuries/metabolism
;
Calcium/metabolism
;
Cells, Cultured
;
Cerebral Cortex/cytology/*metabolism
;
Dizocilpine Maleate/pharmacology
;
Enzyme Inhibitors/pharmacology
;
Excitatory Amino Acid Antagonists/pharmacology
;
Magnesium/*pharmacology
;
Membrane Potentials
;
N-Methylaspartate/*pharmacology
;
Naphthalenes/pharmacology
;
Neurons/cytology/*metabolism
;
Patch-Clamp Techniques
;
Protein Kinase C/antagonists & inhibitors/metabolism
;
Rats
;
Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors/drug effects/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
