Publication Date:
1996-08-09
Description:
The mechanism by which Golgi membrane proteins are retained within the Golgi complex in the midst of a continuous flow of protein and lipid is not yet understood. The diffusional mobilities of mammalian Golgi membrane proteins fused with green fluorescent protein from Aequorea victoria were measured in living HeLa cells with the fluorescence photobleaching recovery technique. The diffusion coefficients ranged from 3 x 10(-9) square centimeters per second to 5 x 10(-9) square centimeters per second, with greater than 90 percent of the chimeric proteins mobile. Extensive lateral diffusion of the chimeric proteins occurred between Golgi stacks. Thus, the chimeras diffuse rapidly and freely in Golgi membranes, which suggests that Golgi targeting and retention of these molecules does not depend on protein immobilization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cole, N B -- Smith, C L -- Sciaky, N -- Terasaki, M -- Edidin, M -- Lippincott-Schwartz, J -- R37 AI14584/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1996 Aug 9;273(5276):797-801.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Building 18T, National Institutes of Health, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8670420" target="_blank"〉PubMed〈/a〉
Keywords:
Aluminum Compounds/pharmacology
;
Diffusion
;
Endoplasmic Reticulum/metabolism
;
Fluorides/pharmacology
;
Golgi Apparatus/*metabolism
;
Green Fluorescent Proteins
;
HeLa Cells
;
Humans
;
Intracellular Membranes/metabolism
;
Luminescent Proteins
;
Mannosidases/*metabolism
;
Membrane Proteins/*metabolism
;
Microscopy, Confocal
;
Mutation
;
N-Acetyllactosamine Synthase/*metabolism
;
Receptors, Peptide/genetics/*metabolism
;
Recombinant Fusion Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
