Publication Date:
1996-02-02
Description:
The Rho guanosine 5'-triphosphatase (GTPase) cycles between the active guanosine triphosphate (GTP)-bound form and the inactive guanosine diphosphate-bound form and regulates cell adhesion and cytokinesis, but how it exerts these actions is unknown. The yeast two-hybrid system was used to clone a complementary DNA for a protein (designated Rhophilin) that specifically bound to GTP-Rho. The Rho-binding domain of this protein has 40 percent identity with a putative regulatory domain of a protein kinase, PKN. PKN itself bound to GTP-Rho and was activated by this binding both in vitro and in vivo. This study indicates that a serine-threonine protein kinase is a Rho effector and presents an amino acid sequence motif for binding to GTP-Rho that may be shared by a family of Rho target proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Watanabe, G -- Saito, Y -- Madaule, P -- Ishizaki, T -- Fujisawa, K -- Morii, N -- Mukai, H -- Ono, Y -- Kakizuka, A -- Narumiya, S -- New York, N.Y. -- Science. 1996 Feb 2;271(5249):645-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, Kyoto University Faculty of Medicine, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8571126" target="_blank"〉PubMed〈/a〉
Keywords:
Adaptor Proteins, Signal Transducing
;
Amino Acid Sequence
;
Animals
;
Cell Line
;
Cloning, Molecular
;
Enzyme Activation
;
GTP Phosphohydrolases/*metabolism
;
GTP-Binding Proteins/chemistry/*metabolism
;
Guanosine Triphosphate/metabolism
;
Humans
;
Membrane Proteins/*metabolism
;
Mice
;
Molecular Sequence Data
;
Phosphorylation
;
Protein Kinase C/chemistry/*metabolism
;
*Protein-Serine-Threonine Kinases
;
Recombinant Fusion Proteins/metabolism
;
Saccharomyces cerevisiae/genetics
;
Signal Transduction
;
ras Proteins
;
*rho GTP-Binding Proteins
;
rhoA GTP-Binding Protein
;
rhoB GTP-Binding Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
