Publication Date:
1996-01-26
Description:
Most nonmalignant cells are anchorage-dependent; they require substrate attachment for growth and, in some instances, survival. This requirement is lost on oncogenic transformation. The cyclin E-CDK2 complex, which is required for the G1-S transition of the cell cycle, was activated in late G1 phase in attached human fibroblasts, but not in fibroblasts maintained in suspension. In transformed fibroblasts the complex was active regardless of attachment. The lack of cyclin E-CDK2 activity in suspended cells appeared to result from increased expression of CDK2 inhibitors and a concomitant decrease in phosphorylation of CDK2 on threonine-160. Suppression of cyclin E-CDK2 activity may thus underlie the anchorage dependence of cell growth.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fang, F -- Orend, G -- Watanabe, N -- Hunter, T -- Ruoslahti, E -- CA 28896/CA/NCI NIH HHS/ -- CA 60725/CA/NCI NIH HHS/ -- CA 67224/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1996 Jan 26;271(5248):499-502.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉La Jolla Cancer Research Foundation, Cancer Center, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8560263" target="_blank"〉PubMed〈/a〉
Keywords:
*CDC2-CDC28 Kinases
;
*Cell Adhesion
;
*Cell Cycle Proteins
;
Cell Line
;
Cell Line, Transformed
;
Cell Nucleus/metabolism
;
*Cell Transformation, Neoplastic
;
Cyclin-Dependent Kinase 2
;
Cyclin-Dependent Kinase Inhibitor p21
;
Cyclin-Dependent Kinase Inhibitor p27
;
Cyclin-Dependent Kinase Inhibitor p57
;
Cyclin-Dependent Kinases/antagonists & inhibitors/*metabolism
;
Cyclins/*metabolism
;
Enzyme Inhibitors/metabolism
;
*G1 Phase
;
Humans
;
Microtubule-Associated Proteins/metabolism
;
Nuclear Proteins/metabolism
;
Phosphorylation
;
Protein-Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
;
Tumor Cells, Cultured
;
*Tumor Suppressor Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
