Publication Date:
1993-10-22
Description:
Several fission yeast temperature-sensitive mutants defective in pre-mRNA processing (prp- mutants) at the nonpermissive temperature have been identified. Here, the prp2+ gene has been cloned by its ability to complement the temperature-sensitive growth defect of a prp2- mutant. The gene also corrects the pre-mRNA splicing defect of prp2- mutants and encodes a 59-kilodalton polypeptide (PRP2). A molecular characterization indicates that PRP2 is a previously uncharacterized yeast splicing factor with extensive similarity to the mammalian splicing factor U2AF65. Thus, this study provides evidence that a U2AF homolog participates in RNA processing in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Potashkin, J -- Naik, K -- Wentz-Hunter, K -- R01GM47487/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1993 Oct 22;262(5133):573-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology and Molecular Biology, University of Health Sciences, Chicago Medical School, IL 60064.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8211184" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Cloning, Molecular
;
Conserved Sequence
;
DEAD-box RNA Helicases
;
Fungal Proteins/chemistry/*genetics/metabolism
;
Genes, Fungal
;
Genetic Complementation Test
;
Molecular Sequence Data
;
*Nuclear Proteins
;
RNA Precursors/*metabolism
;
*RNA Splicing
;
RNA, Fungal/metabolism
;
Ribonucleoproteins/chemistry/*genetics/metabolism
;
*Saccharomyces cerevisiae Proteins
;
Schizosaccharomyces/*genetics
;
Sequence Homology, Amino Acid
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
