Publication Date:
1994-10-14
Description:
Mitogen-activated protein kinases (MAP kinases) are common components of signaling pathways induced by diverse growth stimuli. Although the guanidine nucleotide-binding Ras proteins are known to be upstream activators of MAP kinases, the extent to which MAP kinases directly contribute to the mitogenic effect of Ras is as yet undefined. In this study, inhibition of MAP kinases by the MAP kinase phosphatase MKP-1 blocked the induction of DNA synthesis in quiescent rat embryonic fibroblast REF-52 cells by an activated mutant of Ras, V12Ras. These results suggest an essential role for activation of MAP kinases in the transition from the quiescent to the DNA replication phase of the eukaryotic cell cycle.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sun, H -- Tonks, N K -- Bar-Sagi, D -- CA53840/CA/NCI NIH HHS/ -- CA55360/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1994 Oct 14;266(5183):285-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, NY 11724-2208.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7939666" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Base Sequence
;
Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors
;
*Cell Cycle Proteins
;
Cell Line
;
DNA/*biosynthesis
;
Dual Specificity Phosphatase 1
;
Enzyme Activation
;
G0 Phase
;
HeLa Cells
;
Humans
;
Immediate-Early Proteins/*metabolism/pharmacology
;
JNK Mitogen-Activated Protein Kinases
;
Mitogen-Activated Protein Kinase 1
;
*Mitogen-Activated Protein Kinases
;
Molecular Sequence Data
;
Mutation
;
*Phosphoprotein Phosphatases
;
Protein Phosphatase 1
;
Protein Tyrosine Phosphatases/*metabolism/pharmacology
;
Protein-Serine-Threonine Kinases/*antagonists & inhibitors/metabolism
;
Protein-Tyrosine Kinases/*antagonists & inhibitors/metabolism
;
Rats
;
S Phase
;
Signal Transduction
;
Transfection
;
ras Proteins/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
