Publication Date:
1994-04-01
Description:
The apical ectodermal ridge permits growth and elongation of amniote limb buds; removal causes rapid changes in mesodermal gene expression, patterned cell death, and truncation of the limb. Ectopic fibroblast growth factor (FGF)-2 supplied to the chick apical bud mesoderm after ridge removal will sustain normal gene expression and cell viability, and allow relatively normal limb development. A bioassay for FGFs demonstrated that FGF-2 was the only detectable FGF in chick limb bud extracts. By distribution and bioactivity, FGF-2 is the prime candidate for the chick limb bud apical ridge growth signal.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fallon, J F -- Lopez, A -- Ros, M A -- Savage, M P -- Olwin, B B -- Simandl, B K -- 5T32GM07507/GM/NIGMS NIH HHS/ -- HD20743/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1994 Apr 1;264(5155):104-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Anatomy Department, University of Wisconsin, Madison 53706.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7908145" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Biological Assay
;
Cell Death
;
Cell Differentiation
;
Cell Line
;
Cell Survival
;
Chick Embryo
;
DNA-Binding Proteins/genetics
;
Ectoderm/chemistry/*physiology
;
Extremities/*embryology
;
Fibroblast Growth Factors/analysis/metabolism/pharmacology/*physiology
;
Gene Expression
;
Genes, Homeobox
;
*Homeodomain Proteins
;
Humans
;
MSX1 Transcription Factor
;
Mesoderm/*cytology/metabolism
;
Muscles/cytology
;
Recombinant Proteins/metabolism/pharmacology
;
*Transcription Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
