Publication Date:
1995-05-05
Description:
The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Haq, T A -- Mason, H S -- Clements, J D -- Arntzen, C J -- 1 R01 A136519-01/PHS HHS/ -- New York, N.Y. -- Science. 1995 May 5;268(5211):714-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Plant Biotechnology Program, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University, Houston 77030, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7732379" target="_blank"〉PubMed〈/a〉
Keywords:
Administration, Oral
;
Amino Acid Sequence
;
Animals
;
Bacterial Toxins/immunology
;
Bacterial Vaccines/*administration & dosage/*biosynthesis
;
Base Sequence
;
Enterotoxins/immunology
;
Escherichia coli/immunology
;
*Escherichia coli Proteins
;
Mice
;
Mice, Inbred BALB C
;
Molecular Sequence Data
;
Plants, Genetically Modified/*immunology
;
Plants, Toxic
;
Protein Sorting Signals
;
Recombinant Fusion Proteins/immunology
;
Solanum tuberosum
;
Tobacco
;
Vaccines, Synthetic/*administration & dosage/*biosynthesis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
