Publication Date:
1995-04-14
Description:
One Ras-dependent protein kinase cascade leading from growth factor receptors to the ERK (extracellular signal-regulated kinases) subgroup of mitogen-activated protein kinases (MAPKs) is dependent on the protein kinase Raf-1, which activates the MEK (MAPK or ERK kinase) dual specificity kinases. A second protein kinase cascade leading to activation of the Jun kinases (JNKs) is dependent on MEKK (MEK kinase). A dual-specificity kinase that activates JNK, named JNKK, was identified that functions between MEKK and JNK. JNKK activated the JNKs but did not activate the ERKs and was unresponsive to Raf-1 in transfected HeLa cells. JNKK also activated another MAPK, p38 (Mpk2; the mammalian homolog of HOG1 from yeast), whose activity is regulated similarly to that of the JNKs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lin, A -- Minden, A -- Martinetto, H -- Claret, F X -- Lange-Carter, C -- Mercurio, F -- Johnson, G L -- Karin, M -- New York, N.Y. -- Science. 1995 Apr 14;268(5208):286-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of California-San Diego School of Medicine, La Jolla 92093-0636, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7716521" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Calcium-Calmodulin-Dependent Protein Kinases/*metabolism
;
Cell Line
;
Enzyme Activation
;
Epidermal Growth Factor/pharmacology
;
HeLa Cells
;
Humans
;
JNK Mitogen-Activated Protein Kinases
;
MAP Kinase Kinase 4
;
*MAP Kinase Kinase Kinase 1
;
*Mitogen-Activated Protein Kinase Kinases
;
*Mitogen-Activated Protein Kinases
;
Molecular Sequence Data
;
Phosphorylation
;
Protein Kinases/chemistry/*metabolism
;
Protein-Serine-Threonine Kinases/metabolism
;
Proto-Oncogene Proteins/metabolism
;
Proto-Oncogene Proteins c-raf
;
Recombinant Fusion Proteins/metabolism
;
Substrate Specificity
;
Transfection
;
p38 Mitogen-Activated Protein Kinases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
