Publication Date:
1995-07-28
Description:
T cell hybridomas require the immediate-early gene NGFI-B (nur77) for T cell receptor (TCR)-mediated apoptosis, a model for negative selection of self-reactive T cells. TCR-mediated death was examined in mice bearing an NGFI-B loss-of-function mutation, either by administration of antibodies to CD3 (anti-CD3) or in two well-characterized transgenic models expressing self-reactive TCRs. Both the extent and the rate of thymocyte death were unimpaired. Anti-CD3-induced death was normal in CD4+ peripheral T cells, in which death is mediated predominantly by the Fas signaling pathway. Thus, no unique requirement for NGFI-B is observed for thymic or peripheral T cell death.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lee, S L -- Wesselschmidt, R L -- Linette, G P -- Kanagawa, O -- Russell, J H -- Milbrandt, J -- P01 CA49712/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1995 Jul 28;269(5223):532-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7624775" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies
;
Antigens, CD3/immunology/physiology
;
*Apoptosis
;
Cells, Cultured
;
Clonal Deletion
;
Crosses, Genetic
;
DNA-Binding Proteins/genetics/*physiology
;
Female
;
Gene Targeting
;
Hybridomas
;
Male
;
Mice
;
Mice, Transgenic
;
Nuclear Receptor Subfamily 4, Group A, Member 1
;
Receptors, Antigen, T-Cell, alpha-beta/*physiology
;
Receptors, Cytoplasmic and Nuclear
;
Receptors, Steroid/genetics/*physiology
;
Stem Cells
;
T-Lymphocyte Subsets/cytology
;
T-Lymphocytes/*cytology/immunology
;
Thymus Gland/cytology
;
Transcription Factors/genetics/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
