Publication Date:
1995-10-20
Description:
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Velculescu, V E -- Zhang, L -- Vogelstein, B -- Kinzler, K W -- CA35494/CA/NCI NIH HHS/ -- CA57345/CA/NCI NIH HHS/ -- GM07309/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1995 Oct 20;270(5235):484-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7570003" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Cloning, Molecular
;
DNA Restriction Enzymes
;
DNA, Complementary/genetics
;
*Gene Expression
;
Gene Library
;
*Genetic Techniques
;
Humans
;
Molecular Sequence Data
;
Pancreas/*enzymology
;
Polymerase Chain Reaction
;
RNA, Messenger/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
