Publication Date:
1982-01-01
Description:
The phagocytosis of uniform fluorescent latex particles by pulmonary macrophages in the rat was analyzed by flow cytometric methods. The percentage of phagocytic macrophages and the number of particles per cell were determined from cell-size and fluorescence histograms. A comparison of in vivo and in vitro phagocytosis data showed that the percentage of phagocytic lavaged macrophages reflected the availability of instilled particles. With sodium azide used to model phagocytosis inhibition, it was shown that the percentage of phagocytic cells and the number of particles per cell can be determined simultaneously.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Steinkamp, J A -- Wilson, J S -- Saunders, G C -- Stewart, C C -- AI 15563/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1982 Jan 1;215(4528):64-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7053559" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Flow Cytometry/methods
;
Macrophages/*physiology
;
Microspheres
;
*Phagocytosis
;
Pulmonary Alveoli/cytology
;
Rats
;
Spectrometry, Fluorescence/methods
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
