Publication Date:
1982-12-10
Description:
Tumor induction in athymic nude mice can be used to detect dominant transforming genes in cellular DNA. Mouse NIH 3T3 cells freshly transfected with either cloned Moloney sarcoma proviral DNA or cellular DNA's derived from virally transformed cells induced tumors when injected into athymic nu/nu mice. Tumors were also induced by cells transfected with DNA from two tumor-derived and one chemically transformed human cell lines. The mouse tumors induced by human cell line DNA's contained human DNA sequences, and DNA derived from these tumors was capable of inducing both tumors and foci on subsequent transfection. Tumor induction in nude mice represents a useful new method for the detection and selection of cells transformed by cellular oncogenes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Blair, D G -- Cooper, C S -- Oskarsson, M K -- Eader, L A -- Vande Woude, G F -- New York, N.Y. -- Science. 1982 Dec 10;218(4577):1122-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6293052" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Cell Transformation, Viral
;
DNA, Neoplasm/*genetics
;
DNA, Viral/genetics
;
Mice
;
Mice, Nude/*physiology
;
Moloney murine leukemia virus/genetics
;
Neoplasms, Experimental/*genetics
;
*Oncogenes
;
Sarcoma Viruses, Murine/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
