Publication Date:
1984-09-07
Description:
A growth hormone minigene carrying its natural promoter (237 nucleotides of chromosomal DNA) was stably propagated in a murine retrovirus containing hypoxanthine-guanine phosphoribosyltransferase as a selectable marker. Glucocorticoid and thyroid hormone inducibility was transferred with the growth hormone gene. Recombinant virus with titers of 10(6) per milliliter was recovered. This demonstration that retroviruses can be used to transfer a nonselectable gene under its own regulatory control enlarges the scope of retroviral vectors as potent tools for gene transfer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Miller, A D -- Ong, E S -- Rosenfeld, M G -- Verma, I M -- Evans, R M -- New York, N.Y. -- Science. 1984 Sep 7;225(4666):993-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6089340" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
DNA, Recombinant
;
DNA, Viral/analysis
;
Dexamethasone/pharmacology
;
Gene Expression Regulation
;
*Genes
;
Genes, Viral
;
Genetic Markers
;
*Genetic Vectors
;
Growth Hormone/biosynthesis/*genetics
;
Hypoxanthine Phosphoribosyltransferase/genetics
;
Mice
;
Operon
;
Phenotype
;
RNA, Viral/genetics
;
Rats
;
Retroviridae/*genetics
;
Transcription, Genetic
;
Transfection
;
Triiodothyronine/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
