Publication Date:
1979-10-12
Description:
Embryonic limb-bud mesenchyme was induced to calcify in culture by the addition of 3 mM inorganic phosphate to the medium. Phosphate enhanced calcification of the matrix produced by mesenchymal or fibroblast-like cells, whereas no calcification was evident in areas where cartilage had developed. However, calcification was induced throughout the cell layer by altering the cartilage matrix properties with certain enzymes or by changing the phenotypic expression of the cells with vitamin A.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Binderman, I -- Greene, R M -- Pennypacker, J P -- New York, N.Y. -- Science. 1979 Oct 12;206(4415):222-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/482937" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Bone Development/drug effects
;
Bromodeoxyuridine/pharmacology
;
*Calcification, Physiologic/drug effects
;
Cell Differentiation
;
Cells, Cultured
;
Chick Embryo
;
Collagen/physiology
;
Fibroblasts/cytology
;
Hyaluronoglucosaminidase/metabolism
;
Mesoderm/cytology
;
Phosphates/metabolism
;
Proteoglycans/physiology
;
Vitamin A/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
