Publication Date:
1979-09-28
Description:
Tetramethylrhodamine labeled N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys is a potent chemoattractant for human neutrophils. Binding of this peptide to living neutrophils was observed by means of video intensification microscopy. At 37 degrees C, diffuse membrane fluorescence was seen initially, followed by rapid aggregation and internalization of the fluorescent peptide. These processes are dependent on specific binding to the formal peptide chemotactic receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Niedel, J E -- Kahane, I -- Cuatrecasas, P -- New York, N.Y. -- Science. 1979 Sep 28;205(4413):1412-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/472759" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Membrane/metabolism/ultrastructure
;
*Chemotaxis, Leukocyte
;
Endocytosis
;
Humans
;
In Vitro Techniques
;
Membrane Proteins/metabolism
;
Microscopy, Fluorescence
;
Neutrophils/*physiology
;
Oligopeptides/metabolism
;
Receptors, Drug/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
