Publication Date:
1987-11-06
Description:
The c-erbA gene belongs to a multigene family that encodes transcriptional regulatory proteins including the v-erbA oncogene product, steroid hormone receptors, and the vitamin D3 receptor. A v-erbA DNA probe encoding the DNA-binding region of the v-erbA protein was used to screen a human complementary DNA testis library. One of the clones isolated, erbA-T-1, was found to encode a 490-amino acid protein (erbA-T). The erbA-T polypeptide shows high homology with the proteins encoded by both the chicken c-erbA and the human c-erbA-beta genes but is most closely related to the chicken gene. The chicken c-erbA and the human c-erbA-beta genes encode high-affinity receptors for thyroid hormone, and here it is shown that the erbA-T protein binds specifically to 3,5,3'-triiodo-L-thyronine with a dissociation constant of 3.8 +/- 0.2 x 10(-10) M. These data imply that more than one thyroid hormone receptor exists in humans and that these receptors might have different tissue- and gene-activating specificities.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Benbrook, D -- Pfahl, M -- DK-35083/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1987 Nov 6;238(4828):788-91.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cancer Research Center, La Jolla Cancer Research Foundation, CA 92037.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3672126" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
*Cloning, Molecular
;
DNA/*metabolism
;
*Genes
;
Humans
;
Kinetics
;
Male
;
Protein Biosynthesis
;
*Proto-Oncogenes
;
Receptors, Thyroid Hormone/*genetics/metabolism
;
Testis/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
