Publication Date:
1987-08-28
Description:
Tandem mass spectrometry can be used to solve a number of protein structural problems that are not amenable to conventional methods for amino acid sequencing. Typical problems that use this approach involve characterization of peptides with blocked amino termini or peptides that have been otherwise posttranslationally processed, such as, by phosphorylation or sulfation. The structure and homogeneity of synthetic peptides can also be evaluated. Since peptides can be selectively characterized in the presence of other peptides or contaminants, the need for extensive purification is reduced or eliminated.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Biemann, K -- Scoble, H A -- GM05472/GM/NIGMS NIH HHS/ -- RR00317/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1987 Aug 28;237(4818):992-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3303336" target="_blank"〉PubMed〈/a〉
Keywords:
*Amino Acid Sequence
;
Amino Acyl-tRNA Synthetases
;
Escherichia coli
;
Humans
;
*Mass Spectrometry
;
Phosphorylation
;
Protein Processing, Post-Translational
;
Proteins
;
Saccharomyces cerevisiae
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
