Publication Date:
1986-06-06
Description:
A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉DasSarma, S -- Tischer, E -- Goodman, H M -- New York, N.Y. -- Science. 1986 Jun 6;232(4755):1242-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2871626" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
*DNA, Recombinant
;
Escherichia coli/*genetics
;
Genes
;
Genetic Complementation Test
;
Glutamate-Ammonia Ligase/*genetics
;
Medicago sativa/*genetics
;
Molecular Weight
;
Plasmids
;
Promoter Regions, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
