Publication Date:
1988-10-28
Description:
The T cell antigen receptor consists of an antigen-binding heterodimer that is noncovalently associated with at least five CD3 subunits (gamma, delta, epsilon, zeta, and eta). The CD3-zeta chains are either disulfide-linked homodimers (CD3-zeta 2) or disulfide-linked heterodimers with eta (CD3-zeta eta). Variants of a murine antigen-specific T cell hybridoma that express normal amounts of CD3-zeta 2 but decreased amounts of CD3-zeta eta were isolated. When activated, the parental cell line increased both phosphatidylinositol hydrolysis and serine-specific protein kinase activity to a much greater extent than the variants. In contrast, the activation of a tyrosine-specific kinase after stimulation with a cross-linking antibody to CD3 was similar among these cells. There was a positive linear relation between the expression of CD3-zeta eta and phosphoinositide hydrolysis stimulated by the TCR, suggesting a differential coupling of the T cell alpha beta heterodimer to signal transduction mechanisms due to alpha beta association with either CD3-zeta 2 or CD3-zeta eta.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mercep, M -- Bonifacino, J S -- Garcia-Morales, P -- Samelson, L E -- Klausner, R D -- Ashwell, J D -- New York, N.Y. -- Science. 1988 Oct 28;242(4878):571-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biological Response Modifiers Program, National Cancer Institute, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2845582" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens/immunology
;
Antigens, CD3
;
Antigens, Differentiation, T-Lymphocyte/*physiology
;
Cell Line
;
Electrophoresis, Gel, Two-Dimensional
;
Macromolecular Substances
;
*Membrane Proteins
;
Mice
;
Phosphatidylinositols/*metabolism
;
Phosphoproteins/metabolism
;
Phosphorylation
;
Precipitin Tests
;
Protein Kinase C/physiology
;
Receptors, Antigen, T-Cell/*physiology
;
T-Lymphocytes/*physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
