Publication Date:
1989-08-18
Description:
Oligonucleotides that bind to duplex DNA in a sequence-specific manner by triple helix formation offer an approach to the experimental manipulation of sequence-specific protein binding. Micromolar concentrations of pyrimidine oligodeoxyribonucleotides are shown to block recognition of double helical DNA by prokaryotic modifying enzymes and a eukaryotic transcription factor at a homopurine target site. Inhibition is sequence-specific. Oligonucleotides containing 5-methylcytosine provide substantially more efficient inhibition than oligonucleotides containing cytosine. The results have implications for gene-specific repression by oligonucleotides or their analogs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maher, L J 3rd -- Wold, B -- Dervan, P B -- New York, N.Y. -- Science. 1989 Aug 18;245(4919):725-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Biology, California Institute of Technology, Pasadena 91125.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2549631" target="_blank"〉PubMed〈/a〉
Keywords:
5-Methylcytosine
;
Animals
;
Base Sequence
;
Cytosine/analogs & derivatives
;
DNA/*metabolism
;
DNA Restriction Enzymes
;
DNA, Recombinant
;
DNA-Binding Proteins/*antagonists & inhibitors/metabolism
;
Deoxyribonucleases, Type II Site-Specific/metabolism
;
Metallothionein/genetics
;
Methylation
;
Mice
;
Molecular Sequence Data
;
Mutation
;
Nucleic Acid Conformation
;
Oligodeoxyribonucleotides/*pharmacology
;
Plasmids
;
Promoter Regions, Genetic
;
Structure-Activity Relationship
;
Transcription Factors/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
