Publication Date:
1989-02-24
Description:
Branched RNA-linked multicopy single-stranded DNA (msDNA) originally detected in myxobacteria has now been found in a clinical isolate of Escherichia coli. Although lacking homology in the primary structure, the E. coli msDNA is similar in secondary structure to the myxobacterial msDNA's, including the 2',5'-phosphodiester linkage between RNA and DNA. A chromosomal DNA fragment responsible for the production of msDNA was cloned in an E. coli K12 strain; its DNA sequence revealed an open reading frame (ORF) of 586 amino acid residues. The ORF shows sequence similarity with retroviral reverse transcriptases and ribonuclease H. Disruption of the ORF blocked msDNA production, indicating that this gene is essential for msDNA synthesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lampson, B C -- Sun, J -- Hsu, M Y -- Vallejo-Ramirez, J -- Inouye, S -- Inouye, M -- F32 GM11970-01A1/GM/NIGMS NIH HHS/ -- GM26843/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1989 Feb 24;243(4894 Pt 1):1033-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2466332" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Cloning, Molecular
;
DNA Probes
;
DNA Restriction Enzymes
;
DNA, Bacterial/genetics
;
DNA, Single-Stranded/analysis/biosynthesis/*genetics
;
Endoribonucleases/genetics
;
Escherichia coli/enzymology/*genetics
;
Genes, Bacterial
;
HIV/enzymology/genetics
;
Human T-lymphotropic virus 1/enzymology/genetics
;
Molecular Sequence Data
;
Myxococcales/genetics
;
Nucleic Acid Hybridization
;
RNA, Bacterial/analysis/biosynthesis/*genetics
;
RNA-Directed DNA Polymerase/*genetics
;
Retroviridae/*enzymology/genetics
;
Ribonuclease H
;
Sequence Homology, Nucleic Acid
;
Transformation, Bacterial
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
