Publication Date:
1988-12-02
Description:
The nuclear protein encoded by the proto-oncogene c-myb has been hypothesized to play an important role in the process of hematopoiesis, but direct proof of this function has been lacking. To address this issue, normal human bone marrow mononuclear cells were exposed to c-myb sense and antisense synthetic oligodeoxynucleotides, and the effects on hematopoietic colony formation and maturation were examined. Exposure of these cells to c-myb antisense, oligodeoxynucleotides resulted in a decrease in both colony size and number, without apparent effect on the maturation of residual colony cells. Exposure to c-myb sense, or irrelevant antisense, oligonucleotides had no such effect. These results show that (i) c-myb plays a critical role in regulating normal human hematopoiesis and (ii) the combined use of antisense oligodeoxynucleotides and hematopoietic cell culture techniques will provide a powerful tool for studying the role of proteins encoded by proto-oncogenes, or other specific genes, in normal human hematopoiesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gewirtz, A M -- Calabretta, B -- CA 01324/CA/NCI NIH HHS/ -- CA 36896/CA/NCI NIH HHS/ -- CA 46782/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1988 Dec 2;242(4883):1303-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine, University School of Medicine, Philadelphia, PA 19140.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2461588" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Differentiation
;
Cell Division
;
Clone Cells
;
*Hematopoiesis
;
Humans
;
In Vitro Techniques
;
Nuclear Proteins/*physiology
;
Oligodeoxyribonucleotides/chemical synthesis
;
Peroxidase/genetics
;
Proto-Oncogene Proteins/*physiology
;
Proto-Oncogene Proteins c-myb
;
Rna
;
RNA, Antisense
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
