Publication Date:
1987-01-16
Description:
In order to study the rate and form of tubulin transport in cultured neuronal cells, the fluorescence recovery after the photobleaching of a fluorescent tubulin analog has been followed within the neuritic processes of differentiated PC12 cells. In these cells, as in peripheral axons, tubulin is transported in coherent, nondiffusing waves at two different slow rates that are within the range of the slow components a and b of axonal transport measured in vivo. Finally, it appears that most, if not all, of the tubulin analog is moving out these processes. Thus, slow neuroplasmic transport in cultured neuron-like cells is a good model of axonal transport, in which experimental manipulations of the system can be performed that would be difficult in the whole animal.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Keith, C H -- New York, N.Y. -- Science. 1987 Jan 16;235(4786):337-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2432662" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Axonal Transport
;
Biological Transport
;
Neurons/*metabolism
;
Pheochromocytoma
;
Rats
;
Spectrometry, Fluorescence
;
Tubulin/*metabolism
;
Video Recording
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
