Publication Date:
1990-12-07
Description:
A molecular clone of the Xenopus laevis ets-2 gene was isolated from an oocyte complementary DNA library. The amount of messenger RNA (mRNA) in each oocyte or embryo was almost constant during oogenesis and was maintained until the blastula stage of embryonic development, indicating that the observed 3.2-kilobase transcript is a maternal message. The only normal adult tissue in which ets-2 mRNA was detected was the ovary. Injection of antisense oligonucleotides homologous to the ets-2 sequence into oocytes led to degradation of the mRNA and blocked hormone-induced germinal vesicle breakdown. The ets-2 product is thus required for the meiotic maturation of Xenopus oocytes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, Z Q -- Burdett, L A -- Seth, A K -- Lautenberger, J A -- Papas, T S -- New York, N.Y. -- Science. 1990 Dec 7;250(4986):1416-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21702.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2255913" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Division
;
*DNA-Binding Proteins
;
Embryo, Nonmammalian/physiology
;
Female
;
Gene Expression
;
Gene Library
;
Oligonucleotides, Antisense/pharmacology
;
Oocytes/cytology/drug effects/*physiology
;
Oogenesis
;
Protein-Tyrosine Kinases/genetics
;
Proto-Oncogene Protein c-ets-2
;
Proto-Oncogene Proteins/*genetics
;
*Proto-Oncogenes
;
RNA, Messenger/analysis/genetics
;
*Repressor Proteins
;
*Trans-Activators
;
*Transcription Factors
;
Transcription, Genetic
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
