Publication Date:
1979-08-17
Description:
The protein synthesis inhibitor cycloheximide, at a concentration of 0.08 microgram per milliliter, induced flat morphology within 24 to 48 hours and low saturation density in human osteosarcoma cells transformed by Kirsten murine sarcoma virus (Ki-MSV) or N-methyl-N' nitro-N-nitrosoguanidine. Removal of the protein synthesis inhibitor caused both transformed cells to revert to the transformed phenotype. The demonstration of cell-surface antigens, cross-reacted with antiserums induced by extracts of both types of transformed human cells, was dependent on the presence or absence of cycloheximide in the culture medium. The results show that protein synthesis is required to maintain the transformed state in virally or chemically transformed human cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cho, H Y -- Rhim, J S -- New York, N.Y. -- Science. 1979 Aug 17;205(4407):691-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/223242" target="_blank"〉PubMed〈/a〉
Keywords:
Antigens, Surface/analysis
;
Cell Division/drug effects
;
Cell Survival/drug effects
;
Cell Transformation, Neoplastic/*drug effects/pathology
;
Cell Transformation, Viral/*drug effects
;
Cells, Cultured
;
Cycloheximide/*pharmacology
;
*Gammaretrovirus
;
Humans
;
Methylnitronitrosoguanidine
;
Neoplasm Proteins/biosynthesis
;
*Sarcoma Viruses, Murine
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
