Publication Date:
1990-12-07
Description:
Efficient transport of class I major histocompatibility complex molecules to the cell surface requires association of the class I heavy chain with endogenous peptide and the class I light chain, beta 2-microglobulin (beta 2M). A mutant cell line deficient in beta 2M transports low amounts of nonpeptide-associated heavy chains to the cell surface that can associate with exogenously provided beta 2M and synthetic peptide antigens. Normal beta 2M-sufficient cells grown in serum-free media devoid of beta 2M also require an exogenous source of beta 2M to efficiently bind synthetic peptide. Thus, class I molecules on normal cells do not spontaneously bind or exchange peptides.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vitiello, A -- Potter, T A -- Sherman, L A -- AI-25280/AI/NIAID NIH HHS/ -- CA-25803/CA/NCI NIH HHS/ -- CA-49394/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1990 Dec 7;250(4986):1423-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cytel Corporation, La Jolla, CA 92037.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2124002" target="_blank"〉PubMed〈/a〉
Keywords:
Abelson murine leukemia virus/genetics
;
Animals
;
Antibodies
;
Biological Transport
;
Cell Line
;
Cell Membrane/immunology
;
Cell Transformation, Neoplastic
;
Cytotoxicity, Immunologic
;
Histocompatibility Antigens Class I/analysis/*metabolism
;
Immunoglobulin Heavy Chains/metabolism
;
Kinetics
;
Mice
;
Mice, Inbred Strains
;
T-Lymphocytes, Cytotoxic/immunology
;
beta 2-Microglobulin/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
