Publication Date:
1991-02-15
Description:
The hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by phospholipase C yields the second messengers inositol 1,4,5-trisphosphate (InsP3) and 1,2-diacylglycerol. This activity is regulated by a variety of hormones through G protein pathways. However, the specific G protein or proteins involved has not been identified. The alpha subunit of a newly discovered pertussis toxin-insensitive G protein (Gq) has recently been isolated and is now shown to stimulate the activity of polyphosphoinositide-specific phospholipase C (PI-PLC) from bovine brain. Both the maximal activity and the affinity of PI-PLC for calcium ion were affected. These results identify Gq as a G protein that regulates PI-PLC.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smrcka, A V -- Hepler, J R -- Brown, K O -- Sternweis, P C -- GM31954/GM/NIGMS NIH HHS/ -- GM34497/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1991 Feb 15;251(4995):804-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1846707" target="_blank"〉PubMed〈/a〉
Keywords:
Aluminum/pharmacology
;
*Aluminum Compounds
;
Animals
;
Calcium/physiology
;
Cattle
;
Enzyme Activation/drug effects
;
Fluorides/pharmacology
;
GTP-Binding Proteins/drug effects/*physiology
;
Guanosine Diphosphate
;
In Vitro Techniques
;
Phosphatidylinositol Diacylglycerol-Lyase
;
Phosphoinositide Phospholipase C
;
Phosphoric Diester Hydrolases/*metabolism
;
Substrate Specificity
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
