Publication Date:
1978-02-24
Description:
For the membrane sodium-stimulated magnesium-adenosinetriphosphatase of Acholeplasma laidlawii B both the Vmax and Km values in the Michaelis equation very strongly with temperature. Simulations of Arrhenius plots show that an enzyme with a temperature-dependent Km can yield a variety of Arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Silvius, J R -- Read, B D -- McElhaney, R N -- New York, N.Y. -- Science. 1978 Feb 24;199(4331):902-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/146257" target="_blank"〉PubMed〈/a〉
Keywords:
Acholeplasma laidlawii/enzymology
;
Adenosine Triphosphatases/*metabolism
;
Adenosine Triphosphate/metabolism
;
Fatty Acids/pharmacology
;
Kinetics
;
Magnesium/metabolism
;
Membrane Proteins/metabolism
;
Membranes/*enzymology
;
Temperature
;
Thermodynamics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
