Publication Date:
2000-11-10
Description:
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wu-Scharf, D -- Jeong, B -- Zhang, C -- Cerutti, H -- New York, N.Y. -- Science. 2000 Nov 10;290(5494):1159-62.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉School of Biological Sciences and Plant Science Initiative, University of Nebraska-Lincoln, E211 Beadle Center, Post Office Box 880666, Lincoln, NE 68588, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11073454" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Amino Acid Sequence
;
Animals
;
Chlamydomonas reinhardtii/enzymology/*genetics
;
Cloning, Molecular
;
*DNA Transposable Elements
;
*Gene Silencing
;
Humans
;
Molecular Sequence Data
;
RNA/metabolism
;
RNA Helicases/chemistry/*genetics/*metabolism
;
RNA, Messenger/metabolism
;
Retroelements
;
Reverse Transcriptase Polymerase Chain Reaction
;
Sequence Alignment
;
Sequence Homology, Amino Acid
;
*Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
