Publication Date:
2000-11-04
Description:
The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vergunst, A C -- Schrammeijer, B -- den Dulk-Ras, A -- de Vlaam, C M -- Regensburg-Tuink, T J -- Hooykaas, P J -- New York, N.Y. -- Science. 2000 Nov 3;290(5493):979-82.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11062129" target="_blank"〉PubMed〈/a〉
Keywords:
Agrobacterium tumefaciens/genetics/*metabolism/pathogenicity
;
Arabidopsis/genetics/*metabolism/microbiology
;
Bacterial Proteins/*metabolism
;
DNA, Bacterial/genetics/metabolism
;
DNA-Binding Proteins/*metabolism
;
Drug Resistance
;
Integrases/genetics/*metabolism
;
*Ion Channels
;
Kanamycin/pharmacology
;
Plant Roots/metabolism
;
Plants, Genetically Modified
;
Plasmids
;
Polymerase Chain Reaction
;
*Protein Transport
;
Recombinant Fusion Proteins/metabolism
;
*Viral Proteins
;
Virulence
;
*Virulence Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
