Publication Date:
2000-06-17
Description:
Drosophila offers many advantages as an experimental organism. However, in comparison with yeast and mouse, two other widely used eukaryotic model systems, Drosophila suffers from an inability to perform homologous recombination between introduced DNA and the corresponding chromosomal loci. The ability to specifically modify the genomes of yeast and mouse provides a quick and easy way to generate or rescue mutations in genes for which a DNA clone or sequence is available. A method is described that enables analogous manipulations of the Drosophila genome. This technique may also be applicable to other organisms for which gene-targeting procedures do not yet exist.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rong, Y S -- Golic, K G -- R21GM57792/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Jun 16;288(5473):2013-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10856208" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Animals
;
Blotting, Southern
;
Cloning, Molecular
;
DNA Damage
;
DNA Nucleotidyltransferases/metabolism
;
DNA Repair
;
Deoxyribonucleases, Type II Site-Specific/genetics/metabolism
;
Drosophila melanogaster/*genetics
;
Female
;
*Gene Targeting
;
*Genes, Insect
;
In Situ Hybridization
;
Male
;
*Mutagenesis
;
Mutation
;
Point Mutation
;
*Recombination, Genetic
;
Saccharomyces cerevisiae Proteins
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
