Publication Date:
2000-05-12
Description:
A critical function of tumor suppressor p53 is the induction of apoptosis in cells exposed to noxious stresses. We report a previously unidentified pro-apoptotic gene, Noxa. Expression of Noxa induction in primary mouse cells exposed to x-ray irradiation was dependent on p53. Noxa encodes a Bcl-2 homology 3 (BH3)-only member of the Bcl-2 family of proteins; this member contains the BH3 region but not other BH domains. When ectopically expressed, Noxa underwent BH3 motif-dependent localization to mitochondria and interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. We also demonstrate that blocking the endogenous Noxa induction results in the suppression of apoptosis. Noxa may thus represent a mediator of p53-dependent apoptosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Oda, E -- Ohki, R -- Murasawa, H -- Nemoto, J -- Shibue, T -- Yamashita, T -- Tokino, T -- Taniguchi, T -- Tanaka, N -- New York, N.Y. -- Science. 2000 May 12;288(5468):1053-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10807576" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Motifs
;
Amino Acid Sequence
;
Amino Acid Substitution
;
Animals
;
*Apoptosis
;
Caspase 9
;
Caspases/metabolism
;
Cell Line
;
Cells, Cultured
;
DNA Damage
;
Enzyme Activation
;
Gene Expression Profiling
;
Gene Expression Regulation
;
Humans
;
Mice
;
Mitochondria/metabolism
;
Molecular Sequence Data
;
Promoter Regions, Genetic
;
Proto-Oncogene Proteins/metabolism
;
Proto-Oncogene Proteins c-bcl-2/chemistry/*physiology/*secretion
;
RNA, Messenger/genetics/metabolism
;
T-Lymphocytes/metabolism
;
Tumor Suppressor Protein p53/*physiology
;
bcl-2-Associated X Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
