Publication Date:
2000-05-08
Description:
Light signals perceived by the phytochrome family of sensory photoreceptors are transduced to photoresponsive genes by an unknown mechanism. Here, we show that the basic helix-loop-helix transcription factor PIF3 binds specifically to a G-box DNA-sequence motif present in various light-regulated gene promoters, and that phytochrome B binds reversibly to G-box-bound PIF3 specifically upon light-triggered conversion of the photoreceptor to its biologically active conformer. We suggest that the phytochromes may function as integral light-switchable components of transcriptional regulator complexes, permitting continuous and immediate sensing of changes in this environmental signal directly at target gene promoters.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Martinez-Garcia, J F -- Huq, E -- Quail, P H -- New York, N.Y. -- Science. 2000 May 5;288(5467):859-63.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10797009" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/*genetics/metabolism
;
*Arabidopsis Proteins
;
Basic Helix-Loop-Helix Transcription Factors
;
Cell Cycle Proteins/genetics
;
DNA, Plant/genetics/metabolism
;
DNA-Binding Proteins/genetics
;
Gene Expression Profiling
;
*Gene Expression Regulation, Plant
;
Genes, Plant
;
Helix-Loop-Helix Motifs
;
*Light
;
Models, Genetic
;
Oligodeoxyribonucleotides/genetics/metabolism
;
*Photoreceptor Cells
;
Photosynthetic Reaction Center Complex Proteins/metabolism
;
Phytochrome/*metabolism
;
Phytochrome B
;
*Plant Proteins
;
*Promoter Regions, Genetic
;
Recombinant Fusion Proteins/metabolism
;
Signal Transduction
;
Transcription Factors/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
