Publication Date:
2000-03-31
Description:
In Caenorhabditis elegans, the introduction of double-stranded RNA triggers sequence-specific genetic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Grishok, A -- Tabara, H -- Mello, C C -- GM58800/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 31;287(5462):2494-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Program in Molecular Medicine, Department of Cell Biology, University of Massachusetts Cancer Center, Two Biotech Suite 213, 373 Plantation Street, Worcester, MA 01605, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10741970" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Caenorhabditis elegans/*genetics/physiology
;
*Caenorhabditis elegans Proteins
;
Crosses, Genetic
;
DNA Transposable Elements
;
Disorders of Sex Development
;
Female
;
*Gene Silencing
;
*Genes, Helminth
;
Helminth Proteins/genetics/physiology
;
Male
;
Mutation
;
Phenotype
;
RNA, Double-Stranded/*genetics
;
RNA, Helminth/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
