Publication Date:
1999-11-13
Description:
Before fertilization, vertebrate eggs are arrested in metaphase of meiosis II by cytostatic factor (CSF), an activity that requires activation of the mitogen-activated protein kinase (MAPK) pathway. To investigate whether CSF arrest is mediated by the protein kinase p90Rsk, which is phosphorylated and activated by MAPK, a constitutively activated (CA) form of Rsk was expressed in Xenopus embryos. Expression of CA Rsk resulted in cleavage arrest, and cytological analysis showed that arrested blastomeres were in M phase with prominent spindles characteristic of meiotic metaphase. Thus, Rsk appears to be the mediator of MAPK-dependent CSF arrest in vertebrate unfertilized eggs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gross, S D -- Schwab, M S -- Lewellyn, A L -- Maller, J L -- DK28353/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1999 Nov 12;286(5443):1365-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10558992" target="_blank"〉PubMed〈/a〉
Keywords:
3-Phosphoinositide-Dependent Protein Kinases
;
Animals
;
Blastomeres/*cytology/enzymology
;
Enzyme Activation
;
*MAP Kinase Signaling System
;
Meiosis
;
*Metaphase
;
Mitogen-Activated Protein Kinases/*metabolism
;
Oocytes/cytology/enzymology
;
Protein-Serine-Threonine Kinases/metabolism
;
Proto-Oncogene Proteins c-mos/genetics/metabolism
;
RNA, Messenger/genetics
;
Recombinant Proteins/metabolism
;
Ribosomal Protein S6 Kinases/genetics/*metabolism
;
Spindle Apparatus/ultrastructure
;
Xenopus
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
