Publication Date:
1999-11-13
Description:
Persistent activation of p42 mitogen-activated protein kinase (p42 MAPK) during mitosis induces a "cytostatic factor" arrest, the arrest responsible for preventing the parthenogenetic activation of unfertilized eggs. The protein kinase p90 Rsk is a substrate of p42 MAPK; thus, the role of p90 Rsk in p42 MAPK-induced mitotic arrest was examined. Xenopus laevis egg extracts immunodepleted of Rsk lost their capacity to undergo mitotic arrest in response to activation of the Mos-MEK-1-p42 MAPK cascade of protein kinases. Replenishing Rsk-depleted extracts with catalytically competent Rsk protein restored the ability of the extracts to undergo mitotic arrest. Rsk appears to be essential for cytostatic factor arrest.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bhatt, R R -- Ferrell, J E Jr -- GM16415/GM/NIGMS NIH HHS/ -- GM46383/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1999 Nov 12;286(5443):1362-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Pharmacology, Stanford University School of Medicine, Stanford, CA 94305-5332, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10558991" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
CDC2 Protein Kinase/metabolism
;
Cell Extracts
;
Enzyme Activation
;
MAP Kinase Kinase 1
;
MAP Kinase Signaling System
;
Mitogen-Activated Protein Kinase 1/*metabolism
;
Mitogen-Activated Protein Kinase Kinases/metabolism
;
*Mitosis
;
Molecular Sequence Data
;
Ovum/*cytology/enzymology
;
*Protein-Serine-Threonine Kinases
;
Proto-Oncogene Proteins c-mos/*metabolism/pharmacology
;
Ribosomal Protein S6 Kinases/*metabolism
;
Xenopus laevis
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
