Publication Date:
1999-08-24
Description:
Clathrin-mediated endocytosis is initiated by the recruitment of the clathrin adaptor protein AP-2 to the plasma membrane where the membrane protein synaptotagmin is thought to act as a docking site. AP-2 also interacts with endocytic motifs present in other cargo proteins. Peptides with a tyrosine-based endocytic motif stimulated binding of AP-2 to synaptotagmin and enhanced AP-2 recruitment to the plasma membrane of neuronal and non-neuronal cells. This suggests a mechanism by which nucleation of clathrin-coated pits is stimulated by the loading of cargo proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Haucke, V -- De Camilli, P -- CA46128/CA/NCI NIH HHS/ -- NS36252/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1999 Aug 20;285(5431):1268-71.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06510, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10455054" target="_blank"〉PubMed〈/a〉
Keywords:
Adaptor Protein Complex alpha Subunits
;
Adaptor Proteins, Vesicular Transport
;
Animals
;
Binding Sites
;
CHO Cells
;
*Calcium-Binding Proteins
;
Cattle
;
Cell Membrane/metabolism
;
Clathrin/*metabolism
;
Coated Pits, Cell-Membrane/*metabolism
;
Cricetinae
;
*Endocytosis
;
Membrane Glycoproteins/chemistry/*metabolism
;
Membrane Proteins/*metabolism
;
Nerve Tissue Proteins/chemistry/*metabolism
;
Neurons/metabolism
;
Oligopeptides/chemistry/metabolism/*pharmacology
;
Phospholipase D/metabolism
;
Protein Binding
;
Rats
;
Recombinant Fusion Proteins/metabolism
;
Synaptic Membranes/*metabolism
;
Synaptotagmins
;
Tyrosine/chemistry
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
