Publication Date:
1999-07-31
Description:
Blood coagulation capacity increases with age in healthy individuals. Through extensive longitudinal analyses of human factor IX gene expression in transgenic mice, two essential age-regulatory elements, AE5' and AE3', have been identified. These elements are required and together are sufficient for normal age regulation of factor IX expression. AE5', a PEA-3 related element present in the 5' upstream region of the gene encoding factor IX, is responsible for age-stable expression of the gene. AE3', in the middle of the 3' untranslated region, is responsible for age-associated elevation in messenger RNA levels. In a concerted manner, AE5' and AE3' recapitulate natural patterns of the advancing age-associated increase in factor IX gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kurachi, S -- Deyashiki, Y -- Takeshita, J -- Kurachi, K -- AG13283/AG/NIA NIH HHS/ -- HL38644/HL/NHLBI NIH HHS/ -- HL53713/HL/NHLBI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1999 Jul 30;285(5428):739-43.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-0618, USA. kkurachi@umich.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10426997" target="_blank"〉PubMed〈/a〉
Keywords:
*3' Untranslated Regions
;
Aging/blood/*genetics
;
Animals
;
Consensus Sequence
;
DNA Footprinting
;
Dinucleotide Repeats
;
Factor IX/*genetics/metabolism
;
Female
;
*Gene Expression Regulation
;
Genetic Vectors
;
Humans
;
Male
;
Mice
;
Mice, Transgenic
;
RNA, Messenger/genetics/metabolism
;
*Regulatory Sequences, Nucleic Acid
;
Transcription Factors/genetics/metabolism
;
Transcription, Genetic
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
