Publication Date:
1999-03-19
Description:
The role of localized instability of the actin network in specifying axonal fate was examined with the use of rat hippocampal neurons in culture. During normal neuronal development, actin dynamics and instability polarized to a single growth cone before axon formation. Consistently, global application of actin-depolymerizing drugs and of the Rho-signaling inactivator toxin B to nonpolarized cells produced neurons with multiple axons. Moreover, disruption of the actin network in one individual growth cone induced its neurite to become the axon. Thus, local instability of the actin network restricted to a single growth cone is a physiological signal specifying neuronal polarization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bradke, F -- Dotti, C G -- New York, N.Y. -- Science. 1999 Mar 19;283(5409):1931-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Molecular Biology Laboratory, Cell Biology Programme, Meyerhofstrasse 1, 69012 Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10082468" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/metabolism/*physiology
;
Animals
;
Axons/*physiology/ultrastructure
;
*Bacterial Proteins
;
Bacterial Toxins/pharmacology
;
Bicyclo Compounds, Heterocyclic/pharmacology
;
Cell Polarity
;
Cells, Cultured
;
Cytochalasin D/pharmacology
;
GTP Phosphohydrolases/antagonists & inhibitors/metabolism
;
Growth Cones/drug effects/*physiology/ultrastructure
;
Hippocampus
;
Microtubules/physiology/ultrastructure
;
Neurites/*physiology/ultrastructure
;
Phenotype
;
Pseudopodia/drug effects/ultrastructure
;
Rats
;
Signal Transduction
;
Thiazoles/pharmacology
;
Thiazolidines
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
