Publication Date:
2001-02-07
Description:
Intracellular membrane fusion is mediated by the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins. All vesicle transport steps also have an essential requirement for a member of the Sec1 protein family, including the neuronal Munc18-1 (also known as nSec1) in regulated exocytosis. Here, in adrenal chromaffin cells, we expressed a Munc18 mutant with reduced affinity for syntaxin, which specifically modified the kinetics of single-granule exocytotic release events, consistent with an acceleration of fusion pore expansion. Thus, Munc18 functions in a late stage in the fusion process, where its dissociation from syntaxin determines the kinetics of postfusion events.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Fisher, R J -- Pevsner, J -- Burgoyne, R D -- R01 NS36670-01A1/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2001 Feb 2;291(5505):875-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Physiological Laboratory, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11157167" target="_blank"〉PubMed〈/a〉
Keywords:
Action Potentials
;
Adrenal Medulla/cytology
;
Animals
;
Antigens, Surface/metabolism
;
Catecholamines/secretion
;
Cattle
;
Cells, Cultured
;
Chromaffin Cells/*physiology
;
*Exocytosis
;
*Membrane Fusion
;
Munc18 Proteins
;
Mutation
;
Nerve Tissue Proteins/genetics/*metabolism
;
PC12 Cells
;
Phosphorylation
;
Rats
;
Secretory Vesicles/*physiology
;
Syntaxin 1
;
Transfection
;
*Vesicular Transport Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
