Publication Date:
2013-08-03
Description:
The rapid, reductive early divisions of many metazoan embryos are followed by the midblastula transition (MBT), during which the cell cycle elongates and zygotic transcription begins. It has been proposed that the increasing nuclear to cytoplasmic (N/C) ratio is critical for controlling the events of the MBT. We show that four DNA replication factors--Cut5, RecQ4, Treslin, and Drf1--are limiting for replication initiation at increasing N/C ratios in vitro and in vivo in Xenopus laevis. The levels of these factors regulate multiple events of the MBT, including the slowing of the cell cycle, the onset of zygotic transcription, and the developmental activation of the kinase Chk1. This work provides a mechanism for how the N/C ratio controls the MBT and shows that the regulation of replication initiation is fundamental for normal embryogenesis.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898016/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898016/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Collart, Clara -- Allen, George E -- Bradshaw, Charles R -- Smith, James C -- Zegerman, Philip -- 092096/Wellcome Trust/United Kingdom -- 10-0908/Worldwide Cancer Research/United Kingdom -- C6946/A14492/Cancer Research UK/United Kingdom -- U117597140/Medical Research Council/United Kingdom -- New York, N.Y. -- Science. 2013 Aug 23;341(6148):893-6. doi: 10.1126/science.1241530. Epub 2013 Aug 1.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Cambridge CB2 1QN, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23907533" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Blastula/*embryology/metabolism
;
Carrier Proteins/*metabolism
;
Cell Cycle Proteins/*metabolism
;
Chromosomal Proteins, Non-Histone/*metabolism
;
*DNA Replication
;
RecQ Helicases/*metabolism
;
Xenopus Proteins/*metabolism
;
Xenopus laevis/*embryology/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
