Publication Date:
2011-09-24
Description:
Murine leukemia viruses (MLVs), including xenotropic-MLV-related virus (XMRV), have been controversially linked to chronic fatigue syndrome (CFS). To explore this issue in greater depth, we compiled coded replicate samples of blood from 15 subjects previously reported to be XMRV/MLV-positive (14 with CFS) and from 15 healthy donors previously determined to be negative for the viruses. These samples were distributed in a blinded fashion to nine laboratories, which performed assays designed to detect XMRV/MLV nucleic acid, virus replication, and antibody. Only two laboratories reported evidence of XMRV/MLVs; however, replicate sample results showed disagreement, and reactivity was similar among CFS subjects and negative controls. These results indicate that current assays do not reproducibly detect XMRV/MLV in blood samples and that blood donor screening is not warranted.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299483/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299483/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Simmons, Graham -- Glynn, Simone A -- Komaroff, Anthony L -- Mikovits, Judy A -- Tobler, Leslie H -- Hackett, John Jr -- Tang, Ning -- Switzer, William M -- Heneine, Walid -- Hewlett, Indira K -- Zhao, Jiangqin -- Lo, Shyh-Ching -- Alter, Harvey J -- Linnen, Jeffrey M -- Gao, Kui -- Coffin, John M -- Kearney, Mary F -- Ruscetti, Francis W -- Pfost, Max A -- Bethel, James -- Kleinman, Steven -- Holmberg, Jerry A -- Busch, Michael P -- Blood XMRV Scientific Research Working Group (SRWG) -- N01 HB-57181/HB/NHLBI NIH HHS/ -- N01HB57181/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2011 Nov 11;334(6057):814-7. doi: 10.1126/science.1213841. Epub 2011 Sep 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Blood Systems Research Institute and University of California, San Francisco, San Francisco, CA 94118, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21940862" target="_blank"〉PubMed〈/a〉
Keywords:
Antibodies, Viral/blood
;
Blood/*virology
;
Blood Specimen Collection
;
Cell Line
;
Coculture Techniques
;
False Positive Reactions
;
Fatigue Syndrome, Chronic/*virology
;
Female
;
Humans
;
Laboratories
;
Male
;
Polymerase Chain Reaction
;
Reproducibility of Results
;
Retroviridae Infections/virology
;
Reverse Transcriptase Polymerase Chain Reaction
;
Sensitivity and Specificity
;
Viremia
;
Virus Replication
;
Xenotropic murine leukemia virus-related virus/genetics/immunology/*isolation &
;
purification/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
