Publication Date:
2011-12-07
Description:
Most organisms rely on interhomolog crossovers (COs) to ensure proper meiotic chromosome segregation but make few COs per chromosome pair. By monitoring repair events at a defined double-strand break (DSB) site during Caenorhabditis elegans meiosis, we reveal mechanisms that ensure formation of the obligate CO while limiting CO number. We find that CO is the preferred DSB repair outcome in the absence of inhibitory effects of other (nascent) recombination events. Thus, a single DSB per chromosome pair is largely sufficient to ensure CO formation. Further, we show that access to the homolog as a repair template is regulated, shutting down simultaneously for both CO and noncrossover (NCO) pathways. We propose that regulation of interhomolog access limits CO number and contributes to CO interference.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360972/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360972/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rosu, Simona -- Libuda, Diana E -- Villeneuve, Anne M -- R01 GM067268/GM/NIGMS NIH HHS/ -- R01GM67268/GM/NIGMS NIH HHS/ -- T32 GM007790/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2011 Dec 2;334(6060):1286-9. doi: 10.1126/science.1212424.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22144627" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Caenorhabditis elegans/*genetics
;
Chromosome Pairing
;
*Crossing Over, Genetic
;
DNA Breaks, Double-Stranded
;
DNA Repair
;
DNA, Helminth/*genetics
;
*Meiosis
;
Recombination, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
