Publication Date:
2011-11-26
Description:
Different types of cell behavior, including growth, motility, and navigation, require actin proteins to assemble into filaments. Here, we describe a biochemical process that was able to disassemble actin filaments and limit their reassembly. Actin was a specific substrate of the multidomain oxidation-reduction enzyme, Mical, a poorly understood actin disassembly factor that directly responds to Semaphorin/Plexin extracellular repulsive cues. Actin filament subunits were directly modified by Mical on their conserved pointed-end, which is critical for filament assembly. Mical posttranslationally oxidized the methionine 44 residue within the D-loop of actin, simultaneously severing filaments and decreasing polymerization. This mechanism underlying actin cytoskeletal collapse may have broad physiological and pathological ramifications.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612955/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3612955/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hung, Ruei-Jiun -- Pak, Chi W -- Terman, Jonathan R -- DK 091074/DK/NIDDK NIH HHS/ -- F32 DK091074/DK/NIDDK NIH HHS/ -- NS073968/NS/NINDS NIH HHS/ -- R01 NS073968/NS/NINDS NIH HHS/ -- R01 NS073968-01/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2011 Dec 23;334(6063):1710-3. doi: 10.1126/science.1211956. Epub 2011 Nov 24.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Neuroscience and Pharmacology and Neuroscience Graduate Program, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22116028" target="_blank"〉PubMed〈/a〉
Keywords:
Actin Cytoskeleton/chemistry/*metabolism
;
Actins/chemistry/genetics/*metabolism
;
Amino Acid Sequence
;
Animals
;
Cell Adhesion Molecules/metabolism
;
DNA-Binding Proteins/*metabolism
;
Drosophila
;
Drosophila Proteins/chemistry/genetics/*metabolism
;
Methionine/metabolism
;
Models, Molecular
;
Molecular Sequence Data
;
Mutagenesis, Site-Directed
;
NADP/metabolism
;
Nerve Tissue Proteins/metabolism
;
Oxidation-Reduction
;
Protein Processing, Post-Translational
;
Protein Structure, Tertiary
;
Rabbits
;
Semaphorins/metabolism
;
Substrate Specificity
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
