Publication Date:
2011-06-28
Description:
Synthetic biology has advanced the design of genetic devices that can be used to reprogram metabolic activities in mammalian cells. By functionally linking the signal transduction of melanopsin to the control circuit of the nuclear factor of activated T cells, we have designed a synthetic signaling cascade enabling light-inducible transgene expression in different cell lines grown in culture or bioreactors or implanted into mice. In animals harboring intraperitoneal hollow-fiber or subcutaneous implants containing light-inducible transgenic cells, the serum levels of the human glycoprotein secreted alkaline phosphatase could be remote-controlled with fiber optics or transdermally regulated through direct illumination. Light-controlled expression of the glucagon-like peptide 1 was able to attenuate glycemic excursions in type II diabetic mice. Synthetic light-pulse-transcription converters may have applications in therapeutics and protein expression technology.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ye, Haifeng -- Daoud-El Baba, Marie -- Peng, Ren-Wang -- Fussenegger, Martin -- New York, N.Y. -- Science. 2011 Jun 24;332(6037):1565-8. doi: 10.1126/science.1203535.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biosystems Science and Engineering, Eidgenossische Technische Hochschule (ETH) Zurich, Basel, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21700876" target="_blank"〉PubMed〈/a〉
Keywords:
Alkaline Phosphatase/genetics/metabolism
;
Animals
;
Bioreactors
;
Blood Glucose/*metabolism
;
Cell Line
;
Cell Line, Tumor
;
Diabetes Mellitus, Type 2/genetics/*metabolism
;
GPI-Linked Proteins/genetics/metabolism
;
*Gene Expression Regulation
;
Genes, Reporter
;
Genetic Engineering/*methods
;
Glucagon-Like Peptide 1/genetics/metabolism
;
Homeostasis
;
Humans
;
Insulin/blood
;
Isoenzymes/genetics/metabolism
;
*Light
;
Light Signal Transduction
;
Mice
;
NFATC Transcription Factors/genetics/metabolism
;
Optical Fibers
;
Rod Opsins/genetics/metabolism
;
Signal Transduction
;
Synthetic Biology/*methods
;
*Transcription, Genetic
;
Transfection
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
