Publication Date:
2011-05-21
Description:
Transcriptionally silent genes can be marked by histone modifications and regulatory proteins that indicate the genes' potential to be activated. Such marks have been identified in pluripotent cells, but it is unknown how such marks occur in descendant, multipotent embryonic cells that have restricted cell fate choices. We isolated mouse embryonic endoderm cells and assessed histone modifications at regulatory elements of silent genes that are activated upon liver or pancreas fate choices. We found that the liver and pancreas elements have distinct chromatin patterns. Furthermore, the histone acetyltransferase P300, recruited via bone morphogenetic protein signaling, and the histone methyltransferase Ezh2 have modulatory roles in the fate choice. These studies reveal a functional "prepattern" of chromatin states within multipotent progenitors and potential targets to modulate cell fate induction.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128430/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128430/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xu, Cheng-Ran -- Cole, Philip A -- Meyers, David J -- Kormish, Jay -- Dent, Sharon -- Zaret, Kenneth S -- R01 GM062437/GM/NIGMS NIH HHS/ -- R01 GM062437-12/GM/NIGMS NIH HHS/ -- R01 GM067718/GM/NIGMS NIH HHS/ -- R01 GM067718-08/GM/NIGMS NIH HHS/ -- R37 GM036477/GM/NIGMS NIH HHS/ -- R37 GM036477-28/GM/NIGMS NIH HHS/ -- R37GM36477/GM/NIGMS NIH HHS/ -- U01 DK072503/DK/NIDDK NIH HHS/ -- U01 DK072503-05/DK/NIDDK NIH HHS/ -- U01DK072503/DK/NIDDK NIH HHS/ -- U54MH084691/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2011 May 20;332(6032):963-6. doi: 10.1126/science.1202845.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Regenerative Medicine, Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21596989" target="_blank"〉PubMed〈/a〉
Keywords:
Acetylation
;
Animals
;
Bone Morphogenetic Proteins/metabolism
;
Cell Culture Techniques
;
Cell Differentiation
;
Cell Separation
;
Chromatin/*metabolism
;
Chromatin Immunoprecipitation
;
Embryonic Development
;
Embryonic Induction
;
Endoderm/*cytology
;
*Gene Expression Regulation, Developmental
;
Hepatocytes/cytology
;
Histone-Lysine N-Methyltransferase/metabolism
;
Histones/*metabolism
;
Homeodomain Proteins/genetics/metabolism
;
Liver/cytology/*embryology/metabolism
;
Mice
;
Multipotent Stem Cells/*cytology/metabolism
;
Pancreas/cytology/*embryology/metabolism
;
Polycomb Repressive Complex 2
;
Protein Processing, Post-Translational
;
Regulatory Elements, Transcriptional
;
Signal Transduction
;
Trans-Activators/genetics/metabolism
;
Transcription Factors/metabolism
;
p300-CBP Transcription Factors/antagonists & inhibitors/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
