Publication Date:
2010-02-06
Description:
Alternative splicing of pre-mRNA is a prominent mechanism to generate protein diversity, yet its regulation is poorly understood. We demonstrated a direct role for histone modifications in alternative splicing. We found distinctive histone modification signatures that correlate with the splicing outcome in a set of human genes, and modulation of histone modifications causes splice site switching. Histone marks affect splicing outcome by influencing the recruitment of splicing regulators via a chromatin-binding protein. These results outline an adaptor system for the reading of histone marks by the pre-mRNA splicing machinery.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913848/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913848/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Luco, Reini F -- Pan, Qun -- Tominaga, Kaoru -- Blencowe, Benjamin J -- Pereira-Smith, Olivia M -- Misteli, Tom -- MOP-67011/Canadian Institutes of Health Research/Canada -- R01 AG032134/AG/NIA NIH HHS/ -- R01 AG032134-01/AG/NIA NIH HHS/ -- R01 AG032134-02/AG/NIA NIH HHS/ -- R01 AG032134-03/AG/NIA NIH HHS/ -- R01 AG032134-04/AG/NIA NIH HHS/ -- ZIA BC010309-11/Intramural NIH HHS/ -- New York, N.Y. -- Science. 2010 Feb 19;327(5968):996-1000. doi: 10.1126/science.1184208. Epub 2010 Feb 4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20133523" target="_blank"〉PubMed〈/a〉
Keywords:
*Alternative Splicing
;
Cell Line
;
Chromatin/metabolism
;
Epithelial Cells/metabolism
;
Exons
;
Histone-Lysine N-Methyltransferase/genetics/metabolism
;
Histones/*metabolism
;
Humans
;
Male
;
Mesenchymal Stromal Cells/metabolism
;
Polypyrimidine Tract-Binding Protein/metabolism
;
Prostate/cytology
;
Protein Binding
;
RNA Precursors/*metabolism
;
Receptor, Fibroblast Growth Factor, Type 2/genetics
;
Transcription Factors/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
