Publication Date:
2009-09-12
Description:
Bacterial nitric oxide synthases (bNOS) are present in many Gram-positive species and have been demonstrated to synthesize NO from arginine in vitro and in vivo. However, the physiological role of bNOS remains largely unknown. We show that NO generated by bNOS increases the resistance of bacteria to a broad spectrum of antibiotics, enabling the bacteria to survive and share habitats with antibiotic-producing microorganisms. NO-mediated resistance is achieved through both the chemical modification of toxic compounds and the alleviation of the oxidative stress imposed by many antibiotics. Our results suggest that the inhibition of NOS activity may increase the effectiveness of antimicrobial therapy.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929644/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2929644/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gusarov, Ivan -- Shatalin, Konstantin -- Starodubtseva, Marina -- Nudler, Evgeny -- DP1 OD000799/OD/NIH HHS/ -- DP1 OD000799-02/OD/NIH HHS/ -- DP1 OD000799-03/OD/NIH HHS/ -- DP1 OD000799-04/OD/NIH HHS/ -- New York, N.Y. -- Science. 2009 Sep 11;325(5946):1380-4. doi: 10.1126/science.1175439.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19745150" target="_blank"〉PubMed〈/a〉
Keywords:
Acriflavine/metabolism/pharmacology
;
Anti-Bacterial Agents/metabolism/*pharmacology
;
Antibiosis
;
Bacillus anthracis/drug effects/genetics/growth & development/metabolism
;
Bacillus subtilis/drug effects/genetics/growth & development/metabolism
;
Bacteria/*drug effects/genetics/growth & development/*metabolism
;
Cefuroxime/pharmacology
;
Mutation
;
Nitric Oxide/*metabolism/pharmacology
;
Nitric Oxide Synthase/genetics/*metabolism
;
Oxidative Stress
;
Pseudomonas aeruginosa/growth & development/metabolism
;
Pyocyanine/metabolism/pharmacology
;
Reactive Oxygen Species/metabolism
;
Soil Microbiology
;
Staphylococcus aureus/drug effects/genetics/growth & development/metabolism
;
Superoxide Dismutase/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
