Publication Date:
2008-06-21
Description:
A central question in the study of cell proliferation is, what controls cell-cycle transitions? Although the accumulation of mitotic cyclins drives the transition from the G2 phase to the M phase in embryonic cells, the trigger for mitotic entry in somatic cells remains unknown. We report that the synergistic action of Bora and the kinase Aurora A (Aur-A) controls the G2-M transition. Bora accumulates in the G2 phase and promotes Aur-A-mediated activation of Polo-like kinase 1 (Plk1), leading to the activation of cyclin-dependent kinase 1 and mitotic entry. Mechanistically, Bora interacts with Plk1 and controls the accessibility of its activation loop for phosphorylation and activation by Aur-A. Thus, Bora and Aur-A control mitotic entry, which provides a mechanism for one of the most important yet ill-defined events in the cell cycle.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2834883/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2834883/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Seki, Akiko -- Coppinger, Judith A -- Jang, Chang-Young -- Yates, John R -- Fang, Guowei -- GM062852/GM/NIGMS NIH HHS/ -- HL079442/HL/NHLBI NIH HHS/ -- P41 RR011823/RR/NCRR NIH HHS/ -- P41 RR011823-10/RR/NCRR NIH HHS/ -- R01 GM062852-05/GM/NIGMS NIH HHS/ -- R01 HL079442/HL/NHLBI NIH HHS/ -- R01 HL079442-04/HL/NHLBI NIH HHS/ -- RR11823-10/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2008 Jun 20;320(5883):1655-8. doi: 10.1126/science.1157425.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18566290" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Aurora Kinases
;
CDC2 Protein Kinase/metabolism
;
Cell Cycle Proteins/chemistry/*metabolism
;
Cell Line
;
Enzyme Activation
;
Feedback, Physiological
;
G2 Phase
;
HeLa Cells
;
Humans
;
Kinetics
;
*Mitosis
;
Phosphorylation
;
Protein Binding
;
Protein Structure, Tertiary
;
Protein-Serine-Threonine Kinases/chemistry/*metabolism
;
Proto-Oncogene Proteins/chemistry/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Xenopus
;
Xenopus Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
